gbm cell lines  (ATCC)

Journal: Chemical Biology & Drug Design

Article Title: Triazole‐Linked Pyrimidine Nucleoside Dimers: Design, Synthesis, and Applications in Medicinal and Nucleic Acid Chemistry. Part IV

doi: 10.1111/cbdd.70407

Figure Lengend Snippet: The expression of TYMS in diverse human cancers. The expression levels of TYMS in pan‐cancers were performed by GEPIA database. Red boxes – number of tumor samples ( T ). Gray boxes – number of normal samples ( N ). BRCA – breast invasive carcinoma ( T = 1085; N = 291). CESC – cervical squamous cell carcinoma and endocervical adenocarcinoma ( T = 306; N = 13). GBM – glioblastoma multiforme ( T = 163; N = 207). OV – ovarian serous cystadenocarcinoma ( T = 426; N = 88). * p < 0.05.

Article Snippet: GBM cell lines (U‐251 MG, U‐138 MG), SK‐OV‐3 (ovarian cancer cell line), HeLa (cervical cancer cell line), T47D (breast cancer cell line), and non‐cancerous lung fibroblast cell line (MRC‐5) were purchased from ATCC (Manassas, USA).

Techniques: Expressing

Journal: Molecular Therapy Oncology

Article Title: AAV transduction of human glioblastoma cell lines induces perturbation in cell proliferation

doi: 10.1016/j.omton.2026.201216

Figure Lengend Snippet: Differential transduction of human GBM cell lines with AAV vector serotypes (A) Representative images of U-87 MG and patient-derived cell lines transduced with AAV1, 2, 1/2, 6, and 6.2 expressing eGFP and pBS control-treated control. Green, GFP expression; magenta, EdU staining; blue, Hoechst staining. Scale bars, 100 μm. (B) Quantitation of the transduction efficiency for all serotypes in each cell line as determined by GFP expression. All data presented as mean (SD), n = 3 biological replicates.

Article Snippet: The commercial human GBM cell line U-87 MG (ATCC) was plated at 5,000 cells per well in Eagle’s minimal essential medium (EMEM) (ATCC, 30–2003) supplemented with 10% FBS (Gibco, 10091148) and Penicillin-Streptomycin-Glutamine (Gibco, 10378016).

Techniques: Transduction, Plasmid Preparation, Derivative Assay, Expressing, Control, Staining, Quantitation Assay

Journal: Molecular Therapy Oncology

Article Title: AAV transduction of human glioblastoma cell lines induces perturbation in cell proliferation

doi: 10.1016/j.omton.2026.201216

Figure Lengend Snippet: AAV transduced CD44-positive cell populations with greater efficiency (A) Transduction efficiency of AAV1, 2, 1/2, 6, and 6.2 in CD44-positive and -negative cell populations. Two-way ANOVA showed no significant interaction between the effects of AAV serotype and CD44 expression on transduction efficiency in all cell lines. There was a significant main effect of CD44 expression in U-87 MG ( p = 0.007), case 1 ( p = 0.0003), and case 4 ( p = 0.010) but no statistical significance was found following Sidak’s multiple comparisons test. (B) CD44 expression in human GBM cell lines following AAV treatment. (C) Proliferation of the CD44-positive cell population normalized to values from NTC-treated control. Treatments were compared against their respective pBS-treated control. All data presented as mean (SD), n = 3 biological replicates. Statistical significance is shown as ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: The commercial human GBM cell line U-87 MG (ATCC) was plated at 5,000 cells per well in Eagle’s minimal essential medium (EMEM) (ATCC, 30–2003) supplemented with 10% FBS (Gibco, 10091148) and Penicillin-Streptomycin-Glutamine (Gibco, 10378016).

Techniques: Transduction, Expressing, Control

Journal: Molecular Therapy Oncology

Article Title: AAV transduction of human glioblastoma cell lines induces perturbation in cell proliferation

doi: 10.1016/j.omton.2026.201216

Figure Lengend Snippet: Reductions in cell number and proliferation following AAV treatment persisted in the absence of transgene expression (A) Representative images of U-87 MG, cases 2 and 5 transduced with AAV expressing eGFP or no transgene, along with their respective pBS controls. Green, GFP expression; magenta, EdU staining; blue, Hoechst staining. Scale bars, 100 μm. (B) Total cell number and (C) cell proliferation in the 24 h prior to fixation, normalized to values from NTC-treated control. Treatments were compared against their respective pBS-treated control. All data presented as mean (SD), n = 3 biological replicates. Statistical significance is shown as ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

Article Snippet: The commercial human GBM cell line U-87 MG (ATCC) was plated at 5,000 cells per well in Eagle’s minimal essential medium (EMEM) (ATCC, 30–2003) supplemented with 10% FBS (Gibco, 10091148) and Penicillin-Streptomycin-Glutamine (Gibco, 10378016).

Techniques: Expressing, Transduction, Staining, Control

Journal: Molecular Therapy Oncology

Article Title: AAV transduction of human glioblastoma cell lines induces perturbation in cell proliferation

doi: 10.1016/j.omton.2026.201216

Figure Lengend Snippet: AAV treatment induced cell-cycle arrest at G1 in cases 2 and 5 but not U-87 MG (A) Cumulative cytotoxicity was evaluated by LDH release assay 4 days following AAV treatment, normalized to the maximal LDH release from the Triton X-100 control. No statistical significance was detected. (B) Percentage of cells in each cell cycle phase (G0/G1, S, and G2/M) were quantified via DNA content profiling (see also ). All data presented as mean (SD), n = 3 biological replicates. Treatments were compared against their respective pBS-treated control. Statistical significance is shown as G0/G1 phases: ∗∗∗∗ p < 0.0001; S phase: † p < 0.05; G2/M phases: # p < 0.05, ### p < 0.001.

Article Snippet: The commercial human GBM cell line U-87 MG (ATCC) was plated at 5,000 cells per well in Eagle’s minimal essential medium (EMEM) (ATCC, 30–2003) supplemented with 10% FBS (Gibco, 10091148) and Penicillin-Streptomycin-Glutamine (Gibco, 10378016).

Techniques: Lactate Dehydrogenase Assay, Control

Journal: Cancers

Article Title: Piezo1-ATF3-PPP1r15a Axis Transduces Mechanical Stress into Apoptosis in Glioma Under Low-Intensity Focused Ultrasound

doi: 10.3390/cancers18091445

Figure Lengend Snippet: ATF3 is involved in the ERS of GBM cells treated with LIFU. ( A ) Annexin-V/PI bound U251 or LN229 cells were counted. ( B ) The corresponding statistical results of apoptosis rate. ( C ) The expression levels of Bax, Bcl-2 and caspase3 were determined by Western blot. ( D ) The expression levels of ATF3, ATF4 and CHOP were determined by Western blot. ( E ) U251 and LN229 cells were subjected to reverse transcription-quantitative PCR analysis for ATF4. ( F ) U251 and LN229 cells were subjected to reverse transcription-quantitative PCR analysis for CHOP. ( G ) Representative images of JC-1 staining in U251 with different treatments. ( H ) Representative images of JC-1 staining in LN229 with different treatments. The data are presented as the means ± SEM from at least three independent biological replicates. Statistical comparisons among multiple groups (control, LIFU, siATF3, LIFU + siATF3) were performed using two-way ANOVA followed by Šídák’s multiple comparisons test. *** p < 0.001; **** p < 0.0001. ER, endoplasmic reticulum; GBM, glioblastoma; LIFU, low-intensity focused ultrasound. The original Western blot figures can be found in .

Article Snippet: The American Type Culture Collection provided GBM cell lines (U251 and LN229) and the 293T cell line.

Techniques: Expressing, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction, Staining, Control